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Culture-Free Assembly and Host-Attribution of Phage Genomes in Metagenomes

Date:

24th September, 2021 at 7PM East Africa Time // 9AM Pacific Time

Proximity ligation technology captures physical interactions between DNA molecules in 3D using next-generation sequencing. It can be used to determine which DNA molecules were next to each other in physical space and therefore inside the same cell. This information can be used to deconvolve complex microbial communities, by separating DNA sequences into their respective genomes. Importantly, this method captures connections between host and phage genomes in vivo and therefore the host-range of phages can be determined directly in mixed populations.

Our new tool, "ProxiPhage" uses proximity ligation data to de novo reconstruct phage genomes and link them to their hosts directly from microbiome samples. This method routinely recovers large numbers of novel phages from diverse sample types and can also be applied to other mobile elements, such as plasmids. In this talk, we will discuss the technology, how it works, and show both published and unpublished data centered on our microbiome and phage discovery efforts.

Dr. Ivan Liachko Profile Image

Dr. Ivan Liachko

Dr. Liachko is the founder and CEO/CSO of Phase Genomics and one of the inventors of their core technology, applying proximity ligation data to different facets of genomics. He received his B.S. at Brandeis University in 2001, his Ph.D. at Cornell in 2007, and went on to do his postdoctoral work in the Department of Genome Sciences at the University of Washington in Seattle. He has authored more than 40 academic papers and a number of patents in the field of genomics and synthetic biology. He founded Phase Genomics in 2015 and leads the organization in an executive as well as technical roles.